JMJD1A Represses the Development of Cardiomyocyte Hypertrophy by Regulating the Expression of Catalase

Figure 2

JMJD1A knockdown promotes cardiomyocyte hypertrophy of neonatal rat cardiomyocytes. (a) Representative western blot showing the expression of JMJD1A protein in neonatal rat cardiomyocytes transfected with si-Jmjd1a or si-NC. NRCMs were transfected with si-NC or si-Jmjd1a for 36 hours. (b) Jmjd1a knockdown promotes an ISO-induced increase in cardiomyocyte size. Representative images and quantitative results are shown. NRCMs were transfected with si-NC or si-Jmjd1a for 24 hours and then treated with ISO (1 μM) for 48 hours. vs. PBS+si-NC, # and ^## vs. ISO+si-NC. (c) Jmjd1a knockdown promotes ISO-induced protein synthesis in cardiomyocytes. NRCMs were transfected with si-NC or si-Jmjd1a for 24 hours and then treated with ISO (1 μM) for 48 hours. vs. PBS+si-NC, ^## vs. ISO+si-NC. (d) Jmjd1a knockdown promotes ISO-induced expression of hypertrophic fetal genes. NRCMs were transfected with si-NC or si-Jmjd1a for 24 hours and then treated with ISO (1 μM) for 48 hours. vs. PBS+si-NC, ^## vs. ISO+si-NC. (e) Jmjd1a knockdown promotes angiotensin II (Ang II)-induced increase in cardiomyocyte size. Representative images and quantitative results are shown. NRCMs were transfected with si-NC or si-Jmjd1a for 24 hours and then treated with Ang II (1 μM) for 48 hours. vs. PBS+si-NC, ^## vs. Ang II+si-NC. (f) Jmjd1a knockdown promotes Ang II-induced expression of hypertrophic fetal genes. NRCMs were transfected with si-NC or si-Jmjd1a for 24 hours and then treated with Ang II (1 μM) for 48 hours. vs. PBS+si-NC, ^## vs. Ang II+si-NC.

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