JMJD1A Represses the Development of Cardiomyocyte Hypertrophy by Regulating the Expression of Catalase

Figure 3

JMJD1A overexpression represses cardiomyocyte hypertrophy. (a) Representative western blot showing the expression of JMJD1A proteins in NRCMs infected with adenovirus carrying Jmjd1a or control adenovirus for 36 hours. (b) JMJD1A overexpression represses the ISO-induced increase in cardiomyocyte size. Representative images and quantitative results are shown. NRCMs were infected with indicated adenovirus for 24 hours and then treated with ISO (1 μM) for 48 hours. vs. PBS+Ad-Ctrl, ^## vs. ISO+Ad-Ctrl. (c) JMJD1A overexpression represses ISO-induced protein synthesis in cardiomyocytes. NRCMs were infected with indicated adenovirus for 24 hours and then treated with ISO (1 μM) for 48 hours. vs. PBS+Ad-Ctrl, ^## vs. ISO+Ad-Ctrl. (d) JMJD1A overexpression represses ISO-induced expression of hypertrophic fetal genes. NRCMs were infected with indicated adenovirus for 24 hours and then treated with ISO (1 μM) for 48 hours. vs. PBS+Ad-Ctrl, ^## vs. ISO+Ad-Ctrl.

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