Nephroprotective Role of Zhibai Dihuang Wan in Aristolochic Acid-Intoxicated Zebrafish

Figure 2

ZDW dose titration and survival rate analysis in this study. For ZDW dose titration, wild-type (WT; AB strain) embryos were collected, randomly divided into 50 per experimental group, and exposed to either water (no treatment, control: 0 ppm) or water containing ZDW (1000, 500, and 100 ppm, (a)–(c)) and/or 3 ppm AA. All embryos were cultivated in 6-well cell culture plates, and survival rates were determined at 12, 24, 31, and 48 hpf. The - and -axes represent the developmental stages and embryo survival rates, respectively. The cotreatment group (V) was only applied in low-dose ZDW (100 ppm).