Nephroprotective Role of Zhibai Dihuang Wan in Aristolochic Acid-Intoxicated Zebrafish

Figure 3

Kidney phenotypes of zebrafish embryos after prevention of ZDW. Morphological changes of Tg(wt1b:EGFP) embryos after exposure to water (a) or water containing 1000, 500, and 100 ppm of ZDW (b–d). AA-induced malformed kidney phenotypes which included curved pronephric tube (pt) and swollen, asymmetrical distribution of glomerulus (gl) were observed after being exposed with 3 ppm AA (e). Those phenotypic changes could be attenuated by either pretreatment (3 ppm AA; 100 ppm ZDW) (f) or cotreatment (3 ppm AA+100 ppm ZDW) with ZDW (g). Representative figures are shown here. All photos were taken from a dorsal view at the developmental stage of 48 hpf.

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