Nephroprotective Role of Zhibai Dihuang Wan in Aristolochic Acid-Intoxicated Zebrafish

Figure 5

ZDW pretreatment attenuated AA-induced defective pronephric ducts. Immunohistochemical staining of the basolateral marker Na⁺/K⁺-ATPase alpha1 subunit (α6F) was performed on transverse sections of the pronephric tubes from 48 hpf zebrafish embryos after exposure to water (no treatment group, (a)), 3 ppm of AA (b), 10 ppm of ZDW (c), and AA combined with ZDW (pretreatment (ZDW; AA group) (d) or cotreatment (ZDW+AA) (e)). The sections were stained with a monoclonal antibody (α6F) and counterstained with hematoxylin. (a)-(a), (b)-(b), (c)-(c), (d)-(d), and (e)-(e) were different positions of the embryos indicated by red lines shown in (a), (b), (c), (d), and (e), respectively.

(a)

(b)

(c)

(d)

(e)