Establishment and Characteristic Analysis of a Dog Model for Autologous Homologous Cranioplasty

Figure 2

H&E staining and immunostaing of the reimplanted bone slices. (a) The overview of the sample, ▲ denotes the gap between old and new bones. (b-e) The bone marrow cavity with different sizes of blood cells ① and ④, diploe ②, and nourishing blood vessels within the diploe ③. The outermost surface of the skull was the muscle ⑥, followed by the fibrous tissue and periosteum ⑦. Some of the periosteum, together with the nourishing blood vessels, seem to have been embedded in the outer plate of the skull ⑧ and gradually transformed into osteoblasts ⑨ and osteocytes ⑩. A large amount of matured bone cells that are densely distributed can be seen as small dots in the red bone matrix ⑤. (f-g) The CD34-stained deep brown immune complexes deposited in the microbeam of muscle fiber ((f) left), the periosteal blood vessels ((f) right), the mesenchymal vascular in the bone tissue, the bone lacuna, and the trabecular vessels. (h) The CD31-stained brown immune complexes deposited in the mesenchymal vascular in the bone tissue, Haversian system, and trabecular vessels. (i) The bone trabecula composed of bone active calcium labeled by calcein, with morphology similar to that of some positive structures of CD34 and CD31.

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