Effects of Xuefu Zhuyu Decoction on Cell Migration and Ocular Tumor Invasion in Drosophila

Figure 2

XFZYD suppresses cell migration in Drosophila wing discs. (a–g) Fluorescent images of the 3^rd instar larval wing discs. Compared with ptc-GAL4 UAS-GFP control (a and a’), knockdown of scrib along the A/P boundary triggered massive cell migration in the wing pouch, marked by green fluorescent protein (GFP) (b and b’). (c–g) and (c’–g’) are the representative images of the ptc>scrib-IR model when treated with XFZYD at a concentration of 0.0100, 0.0125, 0.0200, 0.0500, or 0.100 g/ml, respectively. Nuclei were labelled with DAPI (4,6-diamidino-2-phenylindole, blue). Anterior is to the left and dorsal up. (a’–g’) are high magnification of the yellow dotted boxed areas in (a–g), respectively. Statistical analysis of the migrating cell number (h), max migrating distance (i), and median migrating distance (j) in indicated groups ( for each genotype). The columns from left to right are (1) ptc-GAL4 UAS-GFP (ptc>GFP)/+, (2) ptc>GFP/UAS-scrib-IR, and (3–7) ptc>GFP/UAS-scrib-IR larva treated with XFZYD aqueous extract at different concentrations. Error bars indicate the standard deviation. One-way ANOVA with Bonferroni’s multiple comparison test was used to compute values: , , and ; ns: no significant difference. Scale bar: 50 μm (a–g and a’–g’).