Effects of Xuefu Zhuyu Decoction on Cell Migration and Ocular Tumor Invasion in Drosophila

Figure 3

XFZYD downregulates MMP1 and β-integrin expression. Merged fluorescence micrographs of the 3^rd instar larval wing discs stained with anti-MMP1 (a–c) or anti-βPS-integrin antibody (d–f) are shown. The individual channels detecting only GFP (green, a’–f’), only MMP1 (red, a”–c”), and only βPS-integrin signal (red, d”–f”). Nuclei (DNA) were labelled with DAPI (4’,6-diamidino-2-phenylindole, blue). (a”’–f”’) Graphs of total intensity sum of each fluorophore with respect to the region of interest (indicated by the yellow solid line) as shown in (a–f), respectively. Fluorescence intensities were measured in pixels using Image-Pro Plus 6.0. Scale bar: 50 μm (a–f).