Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Rubia yunnanensis Diels Based on Transcriptome Data

<div>Specificity of primer pairs for RT-qPCR amplification. The 2% agarose gel electrophoresis shows the expected size of a single band for each candidate reference gene. M represents the DNA size marker. Lane 1–lane 15: GAPDH, ACT2, EF-1α, eIF, TBP, TUB, PP2A, UBCE, SAND, PEPC, MDH, hnRNP, PTBP2, RPL5, and F-box.</div>

BioMed Research International

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Figure 1

Figure 1: Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Rubia yunnanensis Diels Based on Transcriptome Data

Figure 1 | Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Rubia yunnanensis Diels Based on Transcriptome Data 