Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Rubia yunnanensis Diels Based on Transcriptome Data

Figure 6

Relative expression levels of OSBL and ICS using selected reference genes (the most or the least stable reference genes) for normalization under different tissues (root, stem, and leaf) and MeJA stress treatment sets: (a) OSBL expression levels on different tissues; (b) ICS expression levels on different tissues; (c) OSBL expression levels on leaves under MeJA treatment after 1 h, 6 h, 12 h, and 24 h; (d) ICS expression levels on leaves under MeJA treatment after 1 h, 6 h, 12 h, and 24 h. The error bars represent the mean of three biological replicates ± SD.

(a)

(b)

(c)

(d)