Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Rubia yunnanensis Diels Based on Transcriptome Data

Figure 7

RT-qPCR validation of relative expression levels of six putative key genes involved in anthraquinone biosynthesis pathways using hnRNP and TBP as reference genes in the root and stem leaf (SL) of R. yunnanensis. Columns indicate relative expression levels of six genes calculated by the FPKM method (left y-axis) (Additional Table S1); lines indicating the relative expression levels were obtained by RT-qPCR (right y-axis) (2^−ΔΔCt method). Error bars indicate the standard deviation of mean values of three repeats.

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