Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Rubia yunnanensis Diels Based on Transcriptome Data
Table 3
Expression stability values for R. yunnanensis candidate reference genes calculated by BestKeeper.
Rank
Total
PEG
NaCl
CuSO4
H2O2
MeJA
SA
Cold
Hot
Tissue
1
hnRNP
ACT2
F-box
F-box
PP2A
MDH
SAND
ACT2
F-box
UBCE
2.66 ± 0.65
0.23 ± 0.06
0.77 ± 0.18
0.74 ± 0.17
0.49 ± 0.11
1.50 ± 0.39
0.33 ± 0.09
1.07 ± 0.29
0.99 ± 0.23
2.35 ± 0.51
2
TBP
RPL5
RPL5
SAND
PEPC
RPL5
TUB
TBP
eIF
PP2A
3.17 ± 0.73
0.45 ± 0.10
1.09 ± 0.24
1.50 ± 0.41
0.54 ± 0.11
1.88 ± 0.41
0.68 ± 0.18
1.52 ± 0.36
1.11 ± 0.25
2.87 ± 0.72
3
PP2A
TUB
TUB
MDH
MDH
ACT2
hnRNP
UBCE
PEPC
GAPDH
3.24 ± 0.80
0.53 ± 0.14
1.19 ± 0.33
1.79 ± 0.46
0.75 ± 0.16
2.10 ± 0.56
0.75 ± 0.19
1.52 ± 0.37
1.42 ± 0.34
3.36 ± 0.80
4
TUB
eIF
TBP
TBP
F-box
hnRNP
UBCE
PEPC
PTBP2
hnRNP
3.52 ± 0.97
0.74 ± 0.17
1.25 ± 0.29
1.98 ± 0.47
0.80 ± 0.14
2.23 ± 0.55
0.93 ± 0.21
1.76 ± 0.41
1.43 ± 0.34
3.60 ± 0.89
5
RPL5
hnRNP
hnRNP
hnRNP
PTBP2
SAND
TBP
hnRNP
PP2A
PEPC
4.08 ± 0.89
0.75 ± 0.19
1.37 ± 0.33
2.39 ± 0.59
0.99 ± 0.23
2.45 ± 0.64
0.96 ± 0.22
1.94 ± 0.47
1.48 ± 0.36
3.67 ± 0.82
6
SAND
F-box
MDH
ACT2
hnRNP
GAPDH
PP2A
PTBP2
TBP
TBP
4.55 ± 1.19
0.77 ± 1.18
1.44 ± 0.35
2.45 ± 0.63
1.06 ± 0.25
2.59 ± 0.69
1.01 ± 0.24
1.94 ± 0.51
2.14 ± 0.50
4.00 ± 0.92
7
eIF
MDH
SAND
PP2A
ACT2
PTBP2
ACT2
F-box
TUB
MDH
4.58 ± 1.08
1.03 ± 0.25
1.46 ± 0.37
2.63 ± 0.65
1.09 ± 0.27
2.84 ± 0.73
1.46 ± 0.40
2.00 ± 0.47
2.36 ± 0.63
4.12 ± 0.99
8
MDH
SAND
PEPC
eIF
TBP
eIF
eIF
TUB
hnRNP
ACT2
4.73 ± 1.16
1.05 ± 0.27
1.62 ± 0.33
2.69 ± 0.64
1.16 ± 0.26
2.94 ± 0.70
1.64 ± 0.40
2.02 ± 0.58
2.77 ± 0.67
44.94 ± 1.22
9
UBCE
PTBP2
ACT2
GAPDH
TUB
TBP
PEPC
PP2A
RPL5
PTBP2
4.76 ± 1.06
1.08 ± 0.26
1.68 ± 0.42
2.73 ± 0.68
1.17 ± 0.32
3.11 ± 0.74
1.97 ± 0.44
2.03 ± 0.51
2.90 ± 0.65
5.15 ± 1.25
10
PTBP2
UBCE
UBCE
PEPC
UBCE
TUB
F-box
RPL5
EF-1α
RPL5
5.16 ± 1.28
1.37 ± 0.29
1.73 ± 0.37
3.01 ± 0.66
1.33 ± 0.28
3.76 ± 1.06
2.05 ± 0.51
2.10 ± 0.48
2.94 ± 0.57
5.22 ± 1.17
11
ACT2
PP2A
EF-1α
RPL5
RPL5
UBCE
PTBP2
MDH
UBCE
F-box
5.34 ± 1.37
1.38 ± 0.34
1.96 ± 0.36
3.06 ± 0.67
1.44 ± 0.28
4.02 ± 0.94
2.07 ± 0.54
2.48 ± 0.64
3.19 ± 0.75
5.59 ± 1.33
12
PEPC
TBP
PP2A
EF-1α
eIF
EF-1α
EF-1α
EF-1α
SAND
TUB
5.45 ± 1.20
1.48 ± 0.33
2.05 ± 0.52
3.09 ± 0.60
1.50 ± 0.34
4.11 ± 0.83
2.08 ± 0.42
2.53 ± 0.51
3.67 ± 1.03
6.16 ± 1.69
13
EF-1α
GAPDH
PTBP2
UBCE
SAND
F-box
GAPDH
SAND
ACT2
SAND
5.60 ± 1.09
1.84 ± 0.40
2.68 ± 0.65
3.25 ± 0.74
1.85 ± 0.46
4.11 ± 1.09
2.18 ± 0.60
2.89 ± 0.81
3.84 ± 1.07
7.03 ± 1.78
14
F-box
EF-1α
eIF
TUB
GAPDH
PEPC
RPL5
eIF
GAPDH
EF-1α
6.22 ± 1.46
1.87 ± 0.35
3.61 ± 0.84
3.77 ± 1.05
1.94 ± 0.49
4.92 ± 1.10
2.37 ± 0.51
3.02 ± 0.72
4.00 ± 1.09
7.15 ± 1.41
15
GAPDH
PEPC
GAPDH
PTBP2
EF-1α
PP2A
MDH
GAPDH
MDH
eIF
7.09 ± 1.75
1.96 ± 0.40
4.01 ± 0.89
4.46 ± 1.20
2.19 ± 0.39
5.04 ± 1.26
3.14 ± 0.79
3.25 ± 0.83
5.49 ± 1.38
7.53 ± 1.80
Fifteen candidate reference genes are evaluated by the lowest values of the coefficient of variance (CV) and standard deviation (SD), and these values are showed as CV ± SD.
