Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Rubia yunnanensis Diels Based on Transcriptome Data
Table 4
Comprehensive ranking of 15 candidate reference genes integrated by RefFinder.
Rank
Total
PEG
NaCl
CuSO4
H2O2
MeJA
SA
Cold
Hot
Tissue
1
TBP (1.19)
hnRNP (2.34)
EF-1α (1.68)
hnRNP (1.97)
PTBP2 (2.11)
RPL5 (1.68)
TBP (1.97)
RPL5 (2.14)
RPL5 (2.06)
PTBP2 (2.34)
2
hnRNP (3.08)
MDH (3.03)
UBCE (2.45)
RPL5 (2.58)
hnRNP (2.21)
MDH (1.86)
hnRNP (2.45)
PTBP2 (3.56)
hnRNP (2.34)
hnRNP (2.94)
3
EF-1α (3.56)
eIF (3.83)
hnRNP (3.57)
PEPC (3.08)
TBP (2.82)
hnRNP (2.43)
UBCE (2.99)
hnRNP (3.83)
TBP (3.94)
TBP (3.13)
4
PP2A (3.83)
RPL5 (3.87)
TUB (4.00)
MDH (4.40)
MDH (4.47)
ACT2 (3.46)
eIF (3.13)
EF-1α (3.98)
EF-1α (4.28)
EF-1α (4.12)
5
RPL5 (4.23)
TBP (4.43)
TBP (4.40)
EF-1α (4.43)
RPL5 (4.95)
TBP (5.42)
SAND (5.05)
F-box (4.05)
UBCE (5.36)
UBCE (4.23)
6
UBCE (4.82)
EF-1α (5.19)
RPL5 (4.74)
TBP (6.00)
F-box (6.45)
PTBP2 (5.89)
EF-1α (5.48)
PP2A (4.90)
F-box (5.61)
MDH (6.19)
7
MDH (6.45)
ACT2 (6.15)
MDH (7.48)
SAND (6.34)
PEPC (6.84)
EF-1α (7.36)
ACT2 (6.24)
UBCE (6.05)
PP2A (5.73)
F-box (6.21)
8
eIF (6.96)
SAND (6.16)
F-box (7.62)
eIF (7.24)
eIF (7.14)
SAND (7.52)
PEPC (7.65)
PEPC (6.70)
eIF (5.83)
PP2A (6.67)
9
TUB (7.88)
UBCE (7.33)
PEPC (7.98)
F-box (7.62)
ACT2 (7.24)
eIF (7.84)
PP2A (8.57)
ACT2 (6.71)
PTBP2 (6.09)
PEPC (6.93)
10
PTBP2 (9.08)
PTBP2 (8.74)
PP2A (8.21)
ACT2 (8.38)
PP2A (9.06)
TUB (9.93)
TUB (9.06)
TBP (6.83)
TUB (8.71)
RPL5 (6.96)
11
PEPC (10.70)
TUB (10.00)
SAND (9.46)
PP2A (8.57)
TUB (9.19)
GAPDH (10.70)
RPL5 (9.16)
MDH (9.32)
PEPC (10.00)
GAPDH (9.00)
12
SAND (12.20)
F-box (10.60)
ACT2 (11.00)
GAPDH (10.60)
UBCE (9.74)
UBCE (11.20)
PTBP2 (11.50)
TUB (10.50)
SAND (11.00)
ACT2 (10.20)
13
ACT2 (12.20)
PP2A (10.70)
PTBP2 (12.20)
UBCE (12.00)
SAND (11.70)
PEPC (12.20)
F-box (12.50)
eIF (12.50)
GAPDH (12.70)
TUB (12.50)
14
GAPDH (14.20)
GAPDH (12.20)
eIF (13.20)
PTBP2 (13.50)
EF-1α (12.50)
PP2A (14.20)
GAPDH (12.70)
SAND (14.00)
ACT2 (12.70)
eIF (14.00)
15
F-box (14.70)
PEPC (13.50)
GAPDH (14.20)
TUB (14.00)
GAPDH (14.00)
F-box (14.50)
MDH (14.20)
GAPDH (15.00)
MDH (14.20)
SAND (14.70)
RefFinder exploits computational programs (such as BestKeeper, geNorm, Normfinder, or the comparative delta-ct method) to rank and compare candidate reference genes. The values following the genes indicate the geometric mean of the attributed weights measured by this software for the overall final ranking.
