Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis in Rubia yunnanensis Diels Based on Transcriptome Data

Table 5

Six putative key genes involved in the biosynthetic pathways of anthraquinones in R. yunnanensis.

Enzyme name	Abbreviation	EC number	Query ID
Shikimate pathway key genes forming rings A and B
Isochorismate synthase	ICS	5.4.99.6	TRINITY_DN125672_c2_g2
o-Succinylbenzoate-CoA ligase	OSBL	6.2.1.26	TRINITY_DN125216_c2_g1
Isopentenyl-diphosphate isomerase	IPPI	5.3.3.2	TRINITY_DN131965_c2_g1
Terpenoid pathway key genes forming ring C
Acetyl-CoA acetyltransferase	AACT	2.3.1.9	TRINITY_DN130086_c0_g2
3-Hydroxy-3-methylglutaryl-coenzyme A reductase	HMGR	1.1.1.34	TRINITY_DN129754_c4_g1
1-Deoxy-D-xylulose 5-phosphate synthase	DXS	2.2.1.7	TRINITY_DN124410_c3_g1