Galangin Inhibits Cholangiocarcinoma Cell Growth and Metastasis through Downregulation of MicroRNA-21 Expression

Figure 3

Overexpression of miR-21 abrogates galangin-reduced cell proliferation and galangin-induced cell apoptosis in cholangiocarcinoma cells. HCCC9810 or TFK-1 cells were transfected with 100 nM agomir nonspecific control (NC), miR-21 agomir, antagomir NC, or miR-21 antagomir for 24 hours as indicated elsewhere. (a) HCCC9810 cells were treated with galangin (150M) for 24 hours and harvested for real-time PCR analysis of miR-21 expression. independent experiments. (b) HCCC9810 cells were treated with galangin (150M) for 24 hours and harvested for EdU analysis of cell proliferation. independent experiments. Scale: 20 μM. (c) HCCC9810 cells were transfected with 100 nM antagomir NC or miR-21 antagomir and harvested for EdU analysis of cell proliferation. independent experiments. Scale: 20 μM. (d) Real-time PCR analysis of miR-21 expression in antagomir NC or miR-21 antagomir-transfected HCCC9810 cells. independent experiments. FACS analysis of cell apoptosis in galangin (150 μM)-treated HCCC9810 cells (e) or TFK-1 cells (f) for 24 hours. independent experiments. Western blot analysis of Bax, Bcl-2, cleaved caspase 3 and Caspase 3 expression in galangin (150μM)-treated HCCC9810 cells (g) or TFK-1 cells (h) for 24 hours. independent experiments. Values are given as . .

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