Galangin Inhibits Cholangiocarcinoma Cell Growth and Metastasis through Downregulation of MicroRNA-21 Expression

Figure 5

Overexpression of miR-21 increases galangin-reduced PTEN/AKT signaling in cholangiocarcinoma cells. HCCC9810 cells were transfected with 100 nM agomir nonspecific control (NC) or miR-21 agomir for 24 hours. (a) Western blot analysis of PTEN expression and phosphorylation of AKT in galangin (150 μM)-treated HCCC9810 cells for 24 hours. independent experiments. (b) Schema of galangin reduces cholangiocarcinoma cell growth and metastasis. After galangin treatment, it decreases miR-21 expression, led to increased PTEN expression, an effect that reduces phosphorylation of AKT in cholangiocarcinoma cells, resulting in reduced cell growth and metastasis. Values are given as . .

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