Misaponin B Induces G2/M Arrest, Cytokinesis Failure and Impairs Autophagy

<div>(a) Effect of Misaponin B on LC3B conversion and p62 at the protein level in A549 and AsPC-1 cells. Western blot analysis of A549 and AsPC-1 cells were treated with Misaponin B (7.5 to 45 <i>μ</i>M) for 24 h and subjected to western blotting (<i>n</i> = 2). (b, c) Effect of Misaponin B on LC3B at mRNA level. A549 and AsPC-1 cells were treated with Misaponin B for 24 h and subjected to RT-PCR analysis. (d) Transmission electron microscopy (TEM) images showing autophagic vacuoles in Misaponin B-treated A549 cells. Misaponin B-treated A549 cells were photographed by TEM (<i>n</i> = 3).</div>

BioMed Research International

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Figure 2

Figure 2: Misaponin B Induces G2/M Arrest, Cytokinesis Failure and Impairs Autophagy 