Misaponin B Induces G2/M Arrest, Cytokinesis Failure and Impairs Autophagy

Figure 4

Misaponin B disturbed autophagic flux in A549 cells. After transfection with RFP-GFP-LC3 plasmid, A549 cells were treated by Misaponin B and visualized by confocal microscopy. Representative fluorescence images showing the red/green signals from the mRFP-GFP-LC3 construct for measuring the autophagic flux. Nuclei were stained with DAPI (blue). Punctae: RFP+GFP+ (R+G+: early autophagosomes), RFP+GFP− (R+G−: autolysosomes), and total LC3 punctae. Images merged red and green channels; scale bar: 10 μM (n = 2).