Let-7c-3p Regulates Autophagy under Oxidative Stress by Targeting ATG3 in Lens Epithelial Cells

Figure 2

Let-7c-3p attenuated the apoptosis in SRA01/04 cells under oxidative stress. (a, b) The mRNA expression levels of let-7c-3p in SRA01/04 cells infected by let-7c-3p mimics, mimic controls, let-7c-3p inhibitors, or inhibitor controls were detected by real-time PCR. (c) Forty-eight hours after infection, downregulated let-7c-3p and control groups were treated with 50 μM H₂O₂ for 24 h. Flow cytometry was used to analyze apoptosis. (d) Western blot was used to analyze the expression level of Bcl-2 and Bax. The SRA01/04 cells were treated with 50 μM H₂O₂ for 24 h ().

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