High STRN Expression Promotes HCC Invasion and Migration but Not Cell Proliferation or Apoptosis through Facilitating Epithelial-Mesenchymal Transition

Figure 2

Effect of STRN depletion on the proliferation, apoptosis, invasion, and migration capacities of Huh7 cells. (a) Western blot was conducted to detect the inhibitory effect of STRN siRNA in Huh7 cells. (b) Inhibition of STRN in Huh7 cells was confirmed by immunofluorescence assay (original magnification, ×200). (c) The Huh7 cell proliferation potential was measured by MTT assay upon STRN downregulation via siRNA. (d) Cell apoptosis was assessed using flow cytometric analysis in STRN-downregulated Huh7 cells. (e) The invasive ability of Huh7 cells was suppressed by STRN knockdown, as assessed by the transwell invasion assay. Representative bright-field microscopy images are shown (original magnification, ×200). (f) The migratory capacity of Huh7 cells was inhibited by STRN knockdown, as assessed by the wound healing assay (original magnification, ×100). and vs. NC. Abbreviation: NC: negative control.

(a)

(b)

(c)

(d)

(e)

(f)