High STRN Expression Promotes HCC Invasion and Migration but Not Cell Proliferation or Apoptosis through Facilitating Epithelial-Mesenchymal Transition

Figure 3

Effect of STRN overexpression on the proliferation, apoptosis, invasion, and migration capacities of Huh7 cells. (a) Western blot analysis was performed to confirm the overexpression of STRN in Huh7 cells following transfection with pcDNA3.1/hSTRN. (b) Enhanced STRN expression in Huh7 cells transfected with STRN cDNA was confirmed by immunofluorescence assay (original magnification, ×200). (c) Cell proliferation potential was measured by MTT assay in Huh7 cells transfected with STRN cDNA. (d) Cell apoptosis was assessed using flow cytometric analysis in STRN-upregulated Huh7 cells. (e) Transwell invasion assay was conducted to measure the invasive capacity of Huh7 cells overexpressing STRN, and representative bright-field microscopy images are shown (original magnification, ×200). (f) Wound healing assay was performed to investigate the migratory potential of STRN-overexpressed Huh7 cells (original magnification, ×100). and vs. NC. Abbreviations: NC: negative control; OE: overexpression.

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