The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1

Figure 2

Dynamic localization of Borealin and IQGAP1 during mitosis in HeLa cells. (a) HeLa cells synchronized by double-thymidine treatment were fixed and stained with Borealin antibody (green), alpha-tubulin antibody (red), and DAPI (blue) or (b) IQGAP1 antibody (green) and alpha-tubulin antibody (red), and DAPI (blue). Three independent biological replicates were performed.

(a)

(b)