The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1

Figure 4

Borealin depletion distorted the cell’s architecture. (a). HeLa cells were treated with siRNAs against Borealin or control. After 48 h, cell lysates were detected by western blotting with the indicated antibodies. (b). HeLa cells were treated with siRNAs against Borealin or control. After 48 h, cells were fixed and stained with IQGAP antibody (green), alpha-tubulin antibody (red), and DAPI (blue).  μm. (c). The HeLa cell line with stably expressing mCherry-H2B was treated with siRNAs against Borealin or control. After 48 h, time-lapse imaging was used to observe the process of cell division. (d). Statistic diagram of the time required for cells from nuclear envelope breakdown (NEBD) to the anaphase.

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