The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1

Figure 6

IQGAP1 and Borealin codepletion inhibits cytokinesis completion. (a) HeLa cells were treated with siRNAs directed against Borealin and IQGAP1 together or control. After 48 h, cell lysates were detected by western blotting with the indicated antibodies. (b) RNAi-mediated silencing of Borealin and IQGAP1 together induced multinucleation in HeLa cells. After 48 h, cells were fixed and stained with tubulin antibody (green) and DAPI (blue). (c) IQGAP1 and Borealin codepletion inhibits cytokinesis completion. HeLa cells were individually treated with siRNAs against Borealin alone, IQGAP1 alone, or CDCA8 and IQGAP1 together, and were fixed after 72 h. At least 400 cells were counted every time from three independent experiments. Error bars indicate S.D. Error bars represent ±S.E. ; n.s., not statistically significant.

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