Exosomal circLPAR1 Promoted Osteogenic Differentiation of Homotypic Dental Pulp Stem Cells by Competitively Binding to hsa-miR-31

Figure 6

CircLPAR1 in exosomes induced osteogenic differentiation of recipient homotypic DPSC through downregulation of miR-31. (a) ALP assay of miR-31-inhibited DPSCs and circLPAR1-overexpressed DPSCs cultured for 14 days. (b) Alizarin red staining of miR-31-inhibited groups and circLPAR1-overexpressed group cultured for 21 days. (c) The expression level of important osteogenic differentiation-related genes (SATB2, RUNX2, col-1, and OCN) in miR-31-inhibited group, circLPAR1 overexpressed, and EX7 treated for 14 days. DPSCs were detected by Western blot assay.

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