3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity

Figure 3

Morphological observations, cell growth curves, and hepatocyte viability. (a) Morphological observations in each group during liver tissue generation (all scale bars: 100 μm). (b) Cell growth curves and dynamic testing of hepatocyte viability: cell growth curves (i) and viability trends of the four groups (ii). The data are presented as the . Two-way ANOVA: H vs. P: , , ; H vs. S: ^▲, ^▲▲, ^▲▲▲; H vs. M: ^■, ^■■, ^■■■. H: hepatic plate-mimetic group; M: mixed 3D group; S: sandwich group; P: mixed 2D/plate group.

(a)

(b)