3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity

Figure 7

Transcriptome sequencing analysis outcomes. (a) Volcano plots of differentially expressed genes with an absolute (or ) at different time points in the hepatic plate-mimetic 3D culture group (H) and the mixed 3D culture group (M). Gray and blue colors denote genes without significant differences. Red and green indicate significant upregulated and downregulated genes, respectively. (b) KEGG enrichment bubble charts of the top twenty differentially upregulated genes. Pathways with >1.5 enrichment at different time points in the hepatic plate-mimetic 3D culture group (H) and the mixed 3D culture group (M). H1/M1, Day 1; H7/M7, Day 7; H13/M13, Day 13. (c) KEGG enrichment of the bile secretion pathway for differentially upregulated genes between the hepatic plate-mimetic 3D culture group (H) and the mixed 3D culture group (M) on day 7 with >1.5 enrichment. Red denotes a significant difference; yellow denotes a nonsignificant difference but enrichment >1.5; orange denotes a nonsignificant difference but or ≤1.5; purple denotes a nonsignificant difference but or ≤1.3. BA: bile acids; PL: phospholipids. (d) Differentially expressed genes associated with bile secretion selected from sequencing results and KEGG pathway analysis were analyzed by qPCR. The data represent . Two-way ANOVA: comparison between H and M: , , . H: hepatic plate-mimetic group; M: mixed 3D group. (e) qPCR validation and correlation of expressed genes associated with bile secretion after sequencing and KEGG pathway analysis.

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