Research Article
3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity
Table 1
Preparation of the alginate hydrogel solution.
| Solution components | Concentration | Solution components | Concentration |
| Preparation of the alginate hydrogel solution | Alginate | 0.7% g/mL | BSA | 1% g/mL | NaCl | 0.9% g/mL | HEPES | 10 mmol/L | Buffer solution preparation | Dextran | 10% g/mL | HEPES | 10 mmol/L | NaCl | 0.9% g/mL | | | Gelation solution preparation | Dextran | 10% g/mL | NaCl | 0.72% g/mL | BaCl2 | 20 mmol/L | HEPES | 10 mmol/L |
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BSA: bovine serum albumin; HEPES: N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid.
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