3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity

Table 1

Preparation of the alginate hydrogel solution.

Solution components	Concentration	Solution components	Concentration
Preparation of the alginate hydrogel solution
Alginate	0.7% g/mL	BSA	1% g/mL
NaCl	0.9% g/mL	HEPES	10 mmol/L
Buffer solution preparation
Dextran	10% g/mL	HEPES	10 mmol/L
NaCl	0.9% g/mL
Gelation solution preparation
Dextran	10% g/mL	NaCl	0.72% g/mL
BaCl2	20 mmol/L	HEPES	10 mmol/L

BSA: bovine serum albumin; HEPES: N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid.