3D Culture System for Liver Tissue Mimicking Hepatic Plates for Improvement of Human Hepatocyte (C3A) Function and Polarity

Table 3

Hepatocyte polarity markers.

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Structural polarity

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Basal membrane domain (liver sinusoidal face):
NTCP (sodium-taurocholate cotransporting polypeptide): used to extract bile salts from liver sinusoids.

Lateral membrane domain (hepatocyte face):
ZO-1: tight junction formation;
occludin: tight junction formation;
CD147/CE9 (cluster of differentiation 147/extracellular matrix metalloproteinase inducer): induces matrix metalloproteinase secretion.

Apical domain region (bile canaliculi face):
MRP2/ABCC2 (multidrug resistance-associated protein): promotes bile canaliculi formation and structural integrity;
DPPIV (dipeptidyl peptidase-IV): bile canaliculi-specific protein and regulation of extracellular bioactive peptide concentration.

Ultrastructure: cell-cell connections, bile canaliculi network, polarized Golgi apparatus

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