BioMed Research International / 2020 / Article / Fig 2

Research Article

Acidic Compartment Size, Positioning, and Function during Myogenesis and Their Modulation by the Wnt/Beta-Catenin Pathway

Figure 2

Inhibition of acidic compartment function impairs myogenesis and alters the expression of myogenic and lysosomal genes. Chick myogenic cells were grown for 24 hours, treated with Lys05 at 1 μM, and double-labeled with an antibody against desmin and the nuclear dye DAPI (a–d). Several parameters of muscle proliferation and differentiation were analyzed (e). Image (a) shows the three different types of cells found in chick myogenic cultures: fibroblasts (F), myoblasts (Myb), and myotubes (Myot). Note that fibroblasts (F) are negative for desmin and have a larger nucleus (a), compared to myoblasts (Myb). Scale bar in . qPCR was performed for flotillin-1, flotillin-2, LAMP-1, LAMP-2, desmin, and myogenin gene expression (f).

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