PERK Overexpression-Mediated Nrf2/HO-1 Pathway Alleviates Hypoxia/Reoxygenation-Induced Injury in Neonatal Murine Cardiomyocytes via Improving Endoplasmic Reticulum Stress

Figure 3

The cellular proliferative rate was measured by MTT assay. NCMs were divided into the control group and the H/R group. Then, cells in the H/R group were further separated into the following groups: H/R intervention alone, PERK-siRNA transfection followed by H/R administration, HO-1-rAAV9 infection accompanied by H/R intervention, Nrf2-rAAV9 treatment accompanied by H/R intervention, PERK-rAAV9 infection followed by H/R administration, Nrf2-siRNA transfection accompanied by PERK-rAAV9 treatment and then H/R intervention, HO-1-siRNA transfection accompanied by PERK-rAAV9 treatment and then H/R intervention, and HO-1-siRNA treatment followed by PERK-rAAV9 infection and then H/R administration. Data was shown as mean ± SD. (a) vs control group; (b) vs H/R group; (c) vs H/R group; (d) vs PERK-rAAV9 group; (e) vs Nrf2-rAAV9 group.