miR-425-5p Acts as a Molecular Marker and Promoted Proliferation, Migration by Targeting RNF11 in Hepatocellular Carcinoma

Figure 4

miR-425-5p directly targeted and regulated RNF11. (a) Venn diagram displaying miR-425-5p computationally predicted to target RNF11 by TargetScan, Oncomir, and miRWalk. (b) A schematic diagram of miR-425-5p putative binding sites and the corresponding mutant sites of RNF11. (c) RT-qPCR was used to examine the mRNA levels of RNF11 in HCC cells that were cotransfected with pre-miR-425-5p and miR-425-5p inhibitor. (d) The luciferase activity was used to detect the direct effect of miR-425-5p and RNF11. (e) Western blotting analysis was used to examine the protein expression of RNF11 in HCC cells that were cotransfected with pre- miR-425-5p and miR-425-5p inhibitor. , .

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