Irisin Pretreatment Protects Kidneys against Acute Kidney Injury Induced by Ischemia/Reperfusion via Upregulating the Expression of Uncoupling Protein 2

Figure 5

Irisin suppresses cell apoptosis via regulating UCP2. (a) Representative images of Western blotting and quantitative analysis of cleaved caspase 3 levels normalized to β-actin in vitro. (b, c) The rates of apoptosis in an H/R model in tubular cells ( experiments). (d) Western blot illustrating the expression of UCP2 in cells treated with UCP2 siRNA or p-UCP2. (e) Expression of UCP2 in HK-2 cells pretreated with or without irisin after transfection with UCP2 siRNA or nontargeting siRNA ( experiments). (f, g) The rates of apoptosis in an H/R model in tubular cells transfected with UCP2 siRNA or p-UCP2 with or without pretreatment with irisin versus control groups ( experiments). Statistics are displayed as . ; . Abbreviations: CTL: control, with no addition of irisin; H/R: hypoxia/reoxygenation; NS: no significant difference.

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