Research Article
Toll-like-Receptor Gene Polymorphisms in Tunisian Endemic Pemphigus Foliaceus
Table 1
Primary information of genotyped polymorphisms in TLR2, 3, 4, and 7 genes.
| Gene | SNPs | Base change | Localization | Primers | Enzyme |
| TLR2 | rs5743703/04/05/06/07/09 | G/A-C/A-T/C-T/A-T/G-G/A | Exon 1 | F: 5-GGCCAGCAAATTACCTGTGT-3 R: 5-GATCCCAACTAGACAAAGAC-3 | ┼ | rs5743708 | +2477 G/A (Arg753Gln) | Exon 1 | F: 5-GGCCAGCAAATTACCTGTGT-3 R: 5-GATCCCAACTAGACAAAGAC-3 | Pst1 | (GT)n | — | Intron 2 | F: 5-TATCCCCATTCATTCGTTCCATT-3 R:5Hex-GACCCCCAAGACCCACACC-3 | $ | TLR3 | rs5743305 | -8441 T/A | Promotor | F: 5-GGGACAGTCACGTACTCAGGA-3 R:5-GTGGGCTCCAGCTTCAACTA-3 | SpeI | rs3775294 | T/C | Intron 2 | F: 5-CACATGGCTTATCAAACACAC-3 R:5-CAAAGGGTGATAAAAACATCCT-3 | Pst1 | rs3775291 | +1234 G/A (Leu412Phe) | Exon 4 | F: 5-ATCAGTCGTTGAAGGCTTGG-3 R: 5-TGCTCATTCTCCCTTACACAGA-3 | Tfi1 | TLR4 | rs4986790 | +896A/G (Asp299Gly) | Exon 4 | F: 5-GATTAGCATACTTAGACTACTACCTCCATG-3 R: 5-GATCAACTTCTGAAAAAGCATTCCCAC-3 | Nco I | rs4986791 | +1196 C/T (Thr399Ile) | Exon 4 | F: 5-GGTTGCTGTTC CAAAGTGATTTTGGGACAA-3 R: 5-GGAAATCCAGATGTTCTAGTTGTTCTAAGCC-3 | Hinf1 | TLR7 | rs3853839 | C/G | 3UTR | F: 5TTGCTTCCGTGTCATCCAGG3 R: 5ACAGTACTTTGCAGTGCAGATAAA3 | StyI |
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┼: SNPs were genotyped by direct sequencing; $: primers as described previously [ 24] and was genotyped using the automatic genotyping method. |