Neutrophil-Derived Semaphorin 4D Induces Inflammatory Cytokine Production of Endothelial Cells via Different Plexin Receptors in Kawasaki Disease

Figure 4

Sema4D induces IL-1β, IL-6, and IL-8 production of HCAECs via different plexin receptors. (a) Elevated concentrations of IL-1β, IL-6, and IL-8 in the culture supernatant of HCAECs stimulated with different doses of rSema4D. (b) Increased mRNA expression of IL-1β, IL-6, and IL-8 in rSema4D-stimulated HCAECs. , , , and . (c, d) Decreased concentrations in the culture supernatant (c) and descent mRNA expression of IL-1β, IL-6, and IL-8 of HCAECs (d) after transfection with si-plexin B1 and si-plexin B2. rSema4D in (c) and (d) is 1500 ng/ml. and versus the rSema4D treatment group. n.s.: no significant difference with the rSema4D treatment group; control: serum-free HCAECs.

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