Chronic Venous Disease Patients Showed Altered Expression of IGF-1/PAPP-A/STC-2 Axis in the Vein Wall
Table 2
Primary (A) and secondary (B) antibodies used in the immunohistochemical studies performed, showing the dilutions used and the protocol specifications.
(a)
Antigen
Species
Dilution
Provider
Protocol specifications
IGF-1
Rabbit
1 : 100
Abcam (ab263903)
10 mM sodium citrate, before incubation with blocking solution
PAPP-A
Mouse
1 : 500
Abcam (ab52030)
0.1% Triton in PBS, 10 minutes before incubation with blocking solution
