Research Article
Effect of the Deletion of Genes Related to Amino Acid Metabolism on the Production of Higher Alcohols by Saccharomyces cerevisiae
Table 1
Strains and plasmid used in this study.
| | Strains or plasmids | Genotypic or construct | References or origin |
| | AY15 CICC32315 | Commercial Baijiu yeast strain | This lab | | α5 | MATα, haploid yeast strain from AY15 | This lab | | Transformants | | | | △GAT1 | α5△GAT1::loxP-KanMX-loxP | This study | | △AGP1 | α5△AGP1::loxP-KanMX-loxP | This study | | △BAP2 | α5△BAP2::loxP-KanMX-loxP | This study | | △GDH1 | α5△GDH1::loxP-KanMX-loxP | This study | | △ARO80 | α5△ARO80::loxP-KanMX-loxP | This study | | △CAN1 | α5△CAN1::loxP-KanMX-loxP | This study | | △BAT2 | α5△BAT2::loxP-KanMX-loxP | This study | | △GAD1 | α5△gad1::loxP-KanMX-loxP | This study | | Plasmid | | | | pUG6 | Kanr, containing loxP-KanMX-loxP disruption cassette | Güldener et al., 2002 |
|
|
