Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum

Figure 2

The intracellular growth kinetics of F. novicida within D. discoideum. The D. discoideum cells were infected with F. novicida at MOI 5, 10, and 100. At 2, 4, 24, 48, and 72 h after infection, the cells were lysed and suspension was plated on BCYE agar to determine the number of intracellular bacteria. The experiments were performed in triplicate and the error bars represent standard deviations. .