Research Article
Long Noncoding RNA EZR-AS1 Regulates the Proliferation, Migration, and Apoptosis of Human Venous Endothelial Cells via SMYD3
Figure 2
Effects of lncRNA EZR-AS1 on human venous endothelial cell proliferation, migration, and apoptosis. Cells were transfected with (a) lncRNA si-EZR-AS1 (knockdown) or si-control and with pcDNA-EZR-AS1 (overexpression) or pcDNA-control. Untreated cells were used as a control. Posttransfection with si-EZR-AS1 or pcDNA-EZR-AS1, (b) viability, (c, d) migratory ability, and (e) apoptotic rates of the cells were detected by CCK-8 assay, wound healing assay, Transwell assay, and flow cytometry, respectively. Wound healing was quantified, and the number of migratory cells was counted. Magnification, ×100 (migration). All results were expressed as . and vs. si-control; # and ## vs. pcDNA-control.
(a) |
(b) |
(c) |
(d) |
(e) |