Cervical Cytology of Samples with Ureaplasma urealyticum, Ureaplasma parvum, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma hominis, and Neisseria gonorrhoeae Detected by Multiplex PCR

Figure 3

Cervical cytology of samples with multiple pathogens detected by multiplex PCR: (a) Mycoplasma hominis and Trichomonas vaginalis (sample 14); (b) Mycoplasma hominis, Trichomonas vaginalis, and Ureaplasma parvum (sample 12); (c–f) Chlamydia trachomatis and Neisseria gonorrhoeae (sample 16). Numerous polymorphonuclear leukocytes, isolated and in aggregates (“polyballs”) in (a–f). Pear-shaped organisms with eccentrically located nucleus and eosinophilic cytoplasmic granules, consistent with Trichomonas vaginalis (green arrow) and HSIL (red arrow) in (a). Coccobacilli (blue arrow) and spermatozoid (yellow arrow) in (b). (c–f) Numerous polymorphonuclear leukocytes in mucus were better distributed and less abundant in liquid-based (e, f) than in conventional preparation (c, d). Normal microbiota (lactobacilli) is less abundant in liquid-based preparation (e, f) than in conventional preparation (c, d). (f) Lactobacilli (upper right corner, orange arrow). Papanicolaou: (a, e) 200x; (b) 1000x; (c) 100x; (c, f) 400x; (upper right corner) 1000x. (a–d) Conventional cytology; (e, f) liquid-based cytology.

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