Paraquat Exposure Induces Pulmonary Cell Mitophagy by Enhancing the PINK1/Parkin Signaling

Liu, Kaixiang; Zhan, Zhipeng; Gao, Wei; Feng, Jie; Xie, Xisheng

doi:https://doi.org/10.1155/2020/7103105

BioMed Research International

On this page

Abstract Introduction Materials and Methods Results Discussion Data Availability Conflicts of Interest Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2020 | Article ID 7103105 | https://doi.org/10.1155/2020/7103105

Paraquat Exposure Induces Pulmonary Cell Mitophagy by Enhancing the PINK1/Parkin Signaling

Kaixiang Liu,^1,2Zhipeng Zhan,¹Wei Gao,¹Jie Feng,¹and Xisheng Xie¹

Academic Editor: Pankaj K. Bhavsar

Received26 Sept 2019

Revised03 Dec 2019

Accepted11 Dec 2019

Published22 Jan 2020

Abstract

Paraquat (PQ) poisoning can cause oxidative stress, acute lung injury (ALI), and fibrosis. Excess oxidative stress can induce mitophagy. However, whether PQ exposure can induce mitophagy, contributing to the development of ALI and pulmonary fibrosis in vivo has not been clarified. Here, we show that PQ exposure induces ALI and fiber accumulation in the lung of rats in a time-dependent manner, accompanied by upregulating fibronectin (FN) and Collagen I (COL-I) expression. PQ exposure increased mitophagosome formation and PINK1 and Parkin expression in the lungs of rats. Similarly, PQ exposure reduced the viability and mitochondrial membrane potential, but enhanced FN, COL-I, PINK1, and Parkin expression in A549 cells. In contrast, PINK1 silencing significantly mitigated the PQ-upregulated Parkin, FN, and COL-I expression in A549 cells. Hence, PQ exposure induced ALI and fibrosis in rats by enhancing the PINK1/Parkin signaling and profibrotic factor expression in the lungs. Therefore, our findings suggest that the PINK1/Parkin signaling may be new therapeutic targets and may provide new insights in the pathogenesis of PQ-related ALI in rats.

1. Introduction

Paraquat (PQ) is a unique heterocyclic herbicide and widely used in many developing countries in the world. Chemically, PQ is an oxidant and its exposure may cause poisoning, such as acute respiratory distress syndrome (ARDS) and pulmonary fibrosis, by inducing systemic oxidative stress [1]. Unfortunately, patients with PQ poisoning have a high mortality because currently there is no specific antidote or effective therapy for PQ poisoning [2]. Pathophysiologically, PQ enters the body and can accumulate in pulmonary alveolar epithelial cells, which causes oxidative stress-related lung injury and progressive pulmonary fibrosis, leading to development of respiratory failure and multiple organ dysfunctional syndrome (MODS). Subacute PQ poisoning commonly causes progressive pulmonary fibrosis within days and weeks after exposure [3]. However, the pathogenesis of PQ-related progressive pulmonary fibrosis remains poorly understood.

Previous studies have shown that PQ produces numerous oxygen radicals through a series of linkage reactions when PQ enters the body, particularly in alveolar epithelial cells to form reactive oxygen species (ROS) [4]. The high levels of ROS produced by PQ can cause lipid peroxidation and alveolar epithelial cell apoptosis [5]. Furthermore, PQ-induced pulmonary fibrosis can also be attributed to ROS-induced DNA damage, mitochondrial dysfunction, inflammation, and protease imbalance.

Mitochondrial autophagy (mitophagy) is defined as many factors (such as hypoxia, hunger, photosynthesis, oxidative stress, and others) stimulating the selective degradation of defect mitochondria [6]. During the process of mitophagy, the damaged, defective, and aged mitochondrial membrane is depolarized, which recruits the PTEN-induced kinase 1 (PINK1) and Parkin. Subsequently, PINK1 activates Parkin on the outer mitochondrial membrane and the defect mitochondria are wrapped into the phage specifically and degraded through the ubiquitination and lysosomal degradation processes. PQ produces ROS, which damages the mitochondria, leading to altered mitochondrial membrane potential (MMP) and cell apoptosis. However, it is unknown whether PQ induces mitophagy and affects the PINK1/Parkin signaling in alveolar epithelial cells and in the lung of rats.

In this study, we employed a rat model of PQ poisoning and A549 cells to investigate the effect of PQ exposure on the dynamic changes in the lung injury and fibrosis as well as PINK1 and Parkin expression.

2. Materials and Methods

2.1. Materials and Instruments

The special reagents and equipment included a standard paraquat (Sigma-Aldrich, St. Louis, MO, USA); Cytoplasmic Protein Extraction Kit, Mitochondrial membrane potential assay kit with JC-1, and cell counting kit (C2006, Beyotime Biotechnology, Shanghai, China); antibodies anti-PINK1 (ab23707), anti-Parkin (ab77924), anti-Collagen I (ab34710), anti-β-actin (ab119716, abcam, Cambridge, UK), and anti-Fibronectin (66042-1-lg, Sanying, Wuhan, China); other analytical grade of chemical reagents (Aspen, Beijing, China); Flow cytometry (Beckman FC500, Indianapolis, Beckman); Upright Metallurgical Microscope (Nikon 80i, Tokyo, Japan); transmission electron microscope (Hitachi H-600IV, Tokyo, Japan).

2.2. Animals

Male Sprague Dawley (SD) rats at 6 weeks of age and 180–220 g body weight were obtained from the Experimental Animal Center of the North Sichuan Medical College. The rats were housed in a specific pathogen-free facility at consistent temperature of 20–25°C with a cycle of 12-h light/dark. Animals were allowed free-access to food and water ad libitum. The experimental protocol was approved by the Ethics Committee of the North Sichuan Medical College.

2.3. Animal Model and Grouping

The rats were randomized and provided with 5 ml of saline (control, n = 6) or 50 mg/kg PQ (a suitable dose, based on preliminary studies) in 5 ml of saline by gavage (PQ group, n = 36). At 2 h, 12 h, 1 d, 3 d, 7 d, and 14 d after PQ administration, some rats were anesthetized (n = 5–6 per time point) and subjected to thoracotomy. The proximal end of the main bronchus and the distal end of the trachea were ligated. The left lung was washed three times with saline, and both lungs were collected. The left lung tissue (about 1 mm³) was biopsied from individual rats and fixed in 3% glutaraldehyde overnight. The remaining left lung of each rat was fixed in 4% paraformaldehyde at 4°C overnight and paraffin-embedded. The superior and meddle lubes of the right lungs in individual rats were immediately frozen in liquid nitrogen and stored at −80°C.

2.4. Histopathology

The left lung tissue sections (3 μm) were deparaffined, rehydrated, and stained with haemotoxylin and eosin (H&E) and Masson’s trichrome. The sections were photoimaged under a light microscope, and the severities of lung injury and fibrosis in ten visual fields of each section and five sections per animal were scored by pathologists in a blinded manner, as described previously [7].

2.5. Detection of Mitochondrial Membrane Potential (MMP) by Flow Cytometry

The different groups of cells were stained with JC-1 using the specific kit, according to the manufacturer’s instruction, and the MMP in individual samples was analyzed by flow cytometry.

2.6. Western Blotting

The middle lobe of the right lung from individual rats was homogenized, and the mitochondrial proteins were extracted using a mitochondria protein extraction kit, according to the manufacturer’s instructions. After determining the concentrations of total proteins by the BCA method, individual mitochondrial protein samples (15 μg/lane) were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis on 10% gels and transferred onto polyvinylidene difluoride (PVDF) membranes. The membranes were blocked in 5% nonfat dry milk in TBST and incubated with anti-PINK1, anti-Parkin, anti-FN, anti-COL-I, and anti-β-actin at 4°C overnight. After being washed, the bound antibodies were detected with horseradish peroxidase- (HRP-) conjugated secondary antibodies (1 : 500) at room temperature for 2 h and visualized using the enhanced chemiluminescent reagents. The relative levels of target proteins to the control β-actin were determined by densitometric analysis using the ImageJ software.

Similarly, the relative levels of PINK1, Parkin, FN, and COL-I to β-actin expression in the different groups of A549 cells were determined by western blot assay using specific antibodies.

2.7. Transmission Electron Microscopy

Individual lung tissue samples were prefixed with 3% glutaraldehyde, postfixed in 1% osmium tetroxide, dehydrated in acetone, infiltrated in Epox 812, and embedded. The lung tissue semithin sections (50 nm) were stained with uranyl acetate and lead citrate. The sections were examined using a transmission electron microscope.

2.8. Cell Culture

A549 cells were obtained from American Type Culture Collection (ATCC, Manassas, Virginia, USA) and cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS) at 37°C in an incubator of 5% CO₂. When cells reached 60∼70% of confluency in the logarithmic growth phase, the cells were used for subsequent experiments.

2.9. Gene, Plasmids, and Transfection

A549 cells were cultured in 24-well plates overnight and transfected with control siRNA (siN0000001-1-5) or PINK1-specific siRNA (stB0005003A-1-5, Ribo Biotechnologies, China) using the Lipofectamine 2000 (11668019, ThermoFisher Scientific) for three days and exposed to PQ. Twenty-four hours later, the cells were harvested, and the relative levels of Parkin, FN, and COL-I to β-actin expression in individual groups of cells were determined by Western blot.

2.10. Cell Viability

The cell viability was determined using the cell counting kit (CCK-8), according to the manufacturer’s protocol. Briefly, A549 cells (2 × 10⁵/well) were cultured in 96-well plates overnight and treated in quintuplicate with vehicle saline or different concentrations (50, 100, 200, 400, 600, 800, or 1000 μM) of PQ for varying periods (12–72 h). During the last 2-h culture, individual wells were added with CCK-8 solution (10 μL) and the absorbance (A) of individual wells was measured at 450 nm. The wells without cells served as blank control. The cell viability was calculated using the equation:

2.11. Statistical Analysis

Data are expressed as means ± standard deviation (SD). The difference among groups was analyzed by one-way analysis of variance (ANOVA), and the difference between groups was analyzed by Student’s t test using SPSS 17.0 (IBM, Armonk, NY, USA). A value <0.05 was considered statistically significant.

3. Results

3.1. PQ Causes Pulmonary Tissue Damage in Rats

To determine the effect of PQ on the lung injury and fibrosis, SD rats were randomized and provided with vehicle or PQ by gavage. The rats were sacrificed at different time points after PQ administration, and their lung tissues were examined by H&E and Masson’s trichrome staining. Following PQ exposure, four out of 36 rats died on day 3, 5, 9, and 12 after exposure. As shown in Figures 1(a) and 1(b), while there was normal alveolar structure in the control group of rats, there were moderate inflammation and collagen fibers surrounding the alveolar tissues. The alveolar inflammation and collagen fiber accumulation increased with time after PQ administration. On day 14 after PQ administration, there were many collagen fibers accumulated in the alveolar tissues, which disrupted the alveolar structure in rats. Semiquantitative analysis indicated that the severity of alveolitis and pulmonary fibrosis increased with time (Figure 1(c)). To look for additional evidence of fibrosis, the relative levels of FN and COL-I expression in the lungs of different groups of rats were determined by western blot. In comparison with those in the control rats, the levels of FN and COL-I expression in the lungs of rats following PQ exposure increased in a time-dependent manner (Figure 2). Such data indicated that PQ exposure induced acute alveolitis and progressive pulmonary fibrosis in rats.

(a)

(b)

(c)

Figure 1

PQ exposure induces acute alveolitis and pulmonary fibrosis in rats. After PQ exposure, the rats were sacrificed at the indicated time points and their lung tissues were examined by H&E and Masson’s staining. The severities of alveolitis and pulmonary fibrosis in individual rats were scored. Data are representative images (magnification ×200) or expressed as the mean ± SD of each group (n = 5-6) from two separate experiments. (a) The H&E staining. (b) Masson’s trichrome staining. (c) The scores of alveolitis and pulmonary fibrosis in rats.

(a)

(b)

(c)

PQ exposure reduces the MMP and induces mitophagy by activating the PINK1/Parkin signaling in the lung of rats.

Oxidative stress can damage the mitochondria. To understand the consequence of PQ-induced inflammation and fibrosis, we examined mitophagy in the lung cells from the indicated groups of rats by TEM (Figure 3(a)). In comparison with the control cells, there was obviously mitochondrial swelling in alveolar epithelial cells at 24 h after PQ exposure, and the degrees of mitochondrial swelling gradually increased with vacuoles in the mitochondria. Interestingly, there were denatured mitochondria surrounded by a vacuolar bilayer membrane-like structure, forming the typical double-layer wrapped mitophagy in alveolar epithelial cells from the PQ-exposed rats, but not in the control (Figure 3(a)). In addition, western blot analysis indicated that the relative levels of PINK1 and Parkin expression in the lungs from the PQ-exposed rats increased with time (Figures 3(b) and 3(c)). Collectively, such data indicated that PQ exposure induced mitophagy in alveolar epithelial cells by enhancing the PINK1/Parkin expression in the lung of rats.

(a)

(b)

(c)

3.2. PQ Reduces the Viability of A549 Cells

To further understand the role of PQ in the viability and fibrosis, A549 cells were treated with vehicle or different doses of PQ for the indicated time periods and the cell viability and levels of FN and COL-I expression were determined. As shown in Figure 4(a), treatment with different doses of PQ reduced the viability of A549 cells in a dose- and time-dependent manner. The levels of FN and COL-1expression increased in A549 cells in a dose-dependent manner (Figure 4(b)). Flow cytometry analysis revealed that PQ exposure decreased the MMP in A549 cells (Figure 4(c)). Western blot analysis indicated that the relative levels of PINK1 and Parkin expression in the PQ-exposed cells were significantly higher than those in the control cells, and the effects of PQ on enhancing PINK1 and Parkin expression tended to be dose-dependent ( for all, Figure 4(d)). Such data indicated that PQ exposure reduced the viability and stimulated profibrogenic factor expression in A549 cells by enhancing PINK1 and Parkin expression and mitophagy.

(a)

(b)

(c)

Figure 4

PQ exposure causes cytotoxicity against A549 cell by inducing mitophagy. (a) A549 cells were exposed in triplicate to PQ at the indicated doses for varying time periods and their viability was determined by CCK-8 assays. (b) The relative levels of FN and COL-I in individual groups of cells were determined. (c) The MMP of each group of cells was examined by JC-1 staining and flow cytometry. (d) The relative levels of PINK1 and Parkin expression were examined by western blot. Data are representative images or expressed as the mean ± SD of each group of cells from three separate experiments.

3.3. PINK1 Silencing Mitigates the PQ-Increased FN, COL-I, and Parkin Expression in A549 Cells

Finally, we examined the effect of PINK1 silencing on the levels of FN, COL-I, and Parkin expression in A549 cells. We found that transfection with PINK1-specific siRNA, but not the control siRNA, significantly mitigated not only Parkin expression but also attenuated the PQ-up-regulated FN and COL-I expression in A549 cells ( for all, Figure 5). Therefore, induction of mitophagy and fibrosis by PQ may be dependent on enhancing the PINK1-related signaling in A549 cells.

(a)

(b)

(c)

(d)

4. Discussion

PQ is a highly toxic pesticide, and there is currently no specific measure or antidote for evaluation and treatment of PQ toxicity following PQ poisoning. Because PQ acute poisoning can lead to acute multiple organ failure and chronic pulmonary fibrosis and death, the mortality of patients with PQ poisoning is high [8].

We found that PQ exposure caused ALI and pulmonary fiber accumulation in rats, consistent with previous studies [9]. Evidently, many inflammatory infiltrates, obviously alveolar epithelial edema, telangiectasia, and red blood cell leakage occurred in the lung tissues of rats at 2 h after PQ exposure, accompanied by collagen fiber accumulation. These pathogenic changes increased with time. Similarly, PQ exposure also significantly reduced the viability of A549 cells and enhanced their expression of FN and COL-I in a dose- and time-dependent manner.

Mitophagy is a selective autophagy process and is crucial for the maintenance of mitochondrial function in many types of cells [10]. It is well known that PQ can induce oxidative stress and produce ROS, leading to depletion of antioxidants and development of mitophagy and fibrosis [3, 11, 12]. Actually, previous studies have shown that PQ exposure decreased the MMP and increased PINK1 and Parkin expression, leading to mitophagy and apoptosis in A549 cells [13]. In this study, we found that PQ exposure significantly changed the microstructure of mitochondria with typical feature of mitophagosomes and upregulated PINK1 and Parkin expression in the lung of rats. Such novel data suggest that PINK1 may selectively recruit Parkin on the mitochondrial membrane to ubiquitinate mitochondrial proteins [14]. More importantly, PINK1 silencing significantly mitigated the PQ-upregulated FN and COL-I expression in A549 cells. Accordingly, our findings indicated that PQ exposure caused mitophagy in lung epithelial cells by enhancing the PINK1/Parkin signaling in vivo and in vitro. Hence, inhibition of the PINK1/Parkin signaling may be valuable therapeutic strategies for intervention of PQ poisoning.

In summary, our data indicated that PQ exposure induced ALI and progressive fibrosis in the lungs of rats, accompanied by upregulated PINK1 and Parkin expression and mitophagy in the lung cells. Similarly, PQ exposure significantly reduced the viability and MMP, but enhanced FN, COL-I, PINK1, and Parkin expression in A549 cells, which were significantly mitigated by PINK1 silencing. Such data suggest that the PINK1/Parkin signaling may be valuable therapeutic targets for intervention of PQ poisoning.

Data Availability

The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.

Conflicts of Interest

The authors declare no conflicts of interest.

Acknowledgments

This study was supported by a grant from the Bureau of Science and Technology and Intellectual Property Nanchong City, China (Grant no. 15A0017).

References

W. Myung, G. H. Lee, H. H. Won et al., “Paraquat prohibition and change in the suicide rate and methods in South Korea,” PLoS One, vol. 10, no. 6, Article ID e0128980, 2015.
View at: Publisher Site | Google Scholar
R. J. Dinis-Oliveira, J. A. Duarte, A. Sánchez-Navarro, F. Remião, M. L. Bastos, and F. Carvalho, “Paraquat poisonings: mechanisms of lung toxicity, clinical features, and treatment,” Critical Reviews in Toxicology, vol. 38, no. 1, pp. 13–71, 2008.
View at: Publisher Site | Google Scholar
Z. Suntres, “Role of antioxidants in paraquat toxicity,” Toxicology, vol. 180, no. 1, pp. 65–77, 2002.
View at: Publisher Site | Google Scholar
X. Shao and J. H. Chen, “Progress on pathogenesis and treatment of paraquat-induced pulmonary fibrosis,” Journal of Zhejiang University (Medical Science), vol. 43, no. 6, pp. 717–727, 2014.
View at: Google Scholar
J. P. Gray, D. E. Heck, V. Mishin et al., “Paraquat increases cyanide-insensitive respiration in murine lung epithelial cells by activating an NAD(P)H: paraquat oxidoreductase,” Journal of Biological Chemistry, vol. 282, no. 11, pp. 7939–7949, 2007.
View at: Publisher Site | Google Scholar
J. J. Lemasters, “Selective mitochondrial autophagy, or mitophagy, as a targeted defense against oxidative stress, mitochondrial dysfunction, and aging,” Rejuvenation Research, vol. 8, no. 1, pp. 3–5, 2005.
View at: Publisher Site | Google Scholar
S. V. Szapiel, N. A. Elson, J. D. Fulmer, G. W. Hunninghake, and R. G. Crystal, “Bleomycin-induced interstitial pulmonary disease in the nude, athymic mouse,” The American Review of Respiratory Disease, vol. 120, no. 4, pp. 893–899, 1979.
View at: Publisher Site | Google Scholar
I. B. Gawarammana and N. A. Buckley, “Medical management of paraquat ingestion,” British Journal of Clinical Pharmacology, vol. 72, no. 5, pp. 745–757, 2011.
View at: Publisher Site | Google Scholar
A. Bertram, S. S. Haenel, J. Hadem et al., “Tissue concentration of paraquat on day 32 after intoxication and failed bridge to transplantation by extracorporeal membrane oxygenation therapy,” BMC Pharmacology and Toxicology, vol. 14, no. 1, p. 45, 2013.
View at: Publisher Site | Google Scholar
R. J. Youle and D. P. Narendra, “Mechanisms of mitophagy,” Nature Reviews Molecular Cell Biology, vol. 12, no. 1, pp. 9–14, 2011.
View at: Publisher Site | Google Scholar
L. Wei, J. Wang, A. Chen, J. Liu, X. Feng, and L. Shao, “Involvement of PINK1/parkin-mediated mitophagy in ZnO nanoparticle-induced toxicity in BV-2 cells,” International Journal of Nanomedicine, vol. 12, pp. 1891–1903, 2017.
View at: Publisher Site | Google Scholar
X. Wei, Y. Qi, X. Zhang et al., “Cadmium induces mitophagy through ROS-mediated PINK1/Parkin pathway,” Toxicology Mechanisms and Methods, vol. 24, no. 7, pp. 504–511, 2014.
View at: Publisher Site | Google Scholar
D.-Z. Sun, C.-Q. Song, Y.-M. Xu et al., “Involvement of PINK1/Parkin-mediated mitophagy in paraquat- induced apoptosis in human lung epithelial-like A549 cells,” Toxicology in Vitro, vol. 53, pp. 148–159, 2018.
View at: Publisher Site | Google Scholar
S. Rodriguez-Enriquez, I. Kim, R. T. Currin, and J. J. Lemasters, “Tracker dyes to probe mitochondrial autophagy (mitophagy) in rat hepatocytes,” Autophagy, vol. 2, no. 1, pp. 39–46, 2006.
View at: Publisher Site | Google Scholar

Copyright

Copyright © 2020 Kaixiang Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PDF Download Citation

Download other formats

Order printed copies

Views

477

Downloads

967

Citations