Ephrin-A5 Is Involved in Retinal Neovascularization in a Mouse Model of Oxygen-Induced Retinopathy

Figure 2

Intravitreal injection of LV-Efna5-shRNA effectively silenced Efna5 expression in OIR mouse retinas. (a) The protein level of Efna5 in the retina of OIR mice injected with LV-Efna5-shRNA (LV-Efna5), OIR mice, and C57BL/6J mice was determined by western blot analysis. (b) Relative quantification of protein bands from western blot film was performed (representative of three experiments). (c) Immunohistochemistry staining of Efna5 in the retina of OIR mice injected with LV-Efna5-shRNA (LV-Efna5), OIR mice, and C57BL/6J mice. (d) Counts of Efna5⁺ cells determined by IHC analysis ( animals per group). Scale bar represents 50 μm; P: postnatal day; ILM: internal limiting membrane; GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; OS: outer segment; black arrow: Efna5⁺ cells; ; NS: not significant.

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