ERK1/2 Signaling Pathway Activated by EGF Promotes Proliferation, Transdifferentiation, and Migration of Cultured Primary Newborn Rat Lung Fibroblasts

Table 1

Primers employed for RT–qPCR.

Gene	Sequence	Product size
ERK1	Sense 5-GGTAGACGGTTCTGGAATGGAAGG-3; Antisense 5-GTCAGGGAAAATGGGGTGGG-3	128 bp
ERK2	Sense 5-TGTTCCCAAACGCTGACTCCA-3; Antisense 5-AGTCGTCCAGCTCCATGTCAAACT-3	180 bp
α-SMA	Sense 5-AGCCAGTCGCCATCAGGAAC-3; Antisense 5-CCGGAGCCATTGTCACACAC-3	90 bp
GAPDH	Sense 5-GCACCGTCAAGGCTGAGAAC-3; Antisense 5-ATGGTGGTGAAGACGCCAGT-3	142 bp

Notes: ERK: extracellular signal-regulated kinase; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; RT–qPCR: real-time reverse transcriptase quantitative PCR.