Research Article
Overexpression of blaOXA-58 Gene Driven by ISAba3 Is Associated with Imipenem Resistance in a Clinical Acinetobacter baumannii Isolate from Vietnam
Table 3
Relative quantitation of blaOXA-51 and blaOXA-58 mRNA level and β-lactamase activity in five A. baumannii isolates.
| | Isolate | DN050 | TN078 | DN014 | TN341 | TN345 |
| | MIC imipenem (μg·ml-1) | ≥32 | 0.5 | 0.19 | 0.75 | 0.5 |
| | Relative expression of blaOXA-51 | ΔCt | | | | | | | Expression (time) | 1.00
(0.76-1.31) | 0.06
(0.05-0.08) | 2.51
(1.58-3.98) | 0.59
(0.36-0.96) | 1.04
(0.69-1.59) |
| | Relative expression of blaOXA-58 | ΔCt | | | | | | | Expression (time) | 25.69
(11.3-58.37) | 1.17
(0.84-1.64) | 1.84
(0.39-8.61) | 1.33
(0.93-1.93) | 3.01
(2.55-3.56) |
| | Total β-lactamase activity (U·mg-1) | Extracellular | | | | | | | Periplasmic | | | | | |
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