miR-30e-3p Promotes Cardiomyocyte Autophagy and Inhibits Apoptosis via Regulating Egr-1 during Ischemia/Hypoxia

Figure 1

Expression of miR-30e-3p, Egr-1, and key protein involved in autophagy in IH-exposed cardiomyocytes. (a) Viability of IH-exposed cardiomyocytes reduced gradually in a time-dependent manner as determined using MTS assays. (b) Quantitation of LDH secretion levels in IH-exposed cardiomyocytes. (c, d) Expression levels of miR-30e-3p and Egr-1 mRNA measured using RT-qPCR. (e) LC3II and p62 expression determined by western blot analysis. (f, g) Quantification of LC3II and p62 protein levels. (h) Egr-1 protein expression determined by western blot analysis. (i) Quantification of Egr-1 protein levels (; and , compared to the 0 h group). h: hour; LDH: lactate dehydrogenase.

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