BioMed Research International / 2020 / Article / Fig 4

Research Article

miR-30e-3p Promotes Cardiomyocyte Autophagy and Inhibits Apoptosis via Regulating Egr-1 during Ischemia/Hypoxia

Figure 4

Overexpression of miR-30e-3p promotes autophagy and alleviates cardiomyocyte injury by regulating Egr-1 in IH-exposed cardiomyocytes. (a) Viability of cardiomyocytes determined using MTS assays. (b) Quantitation of LDH secretion levels in IH-exposed cardiomyocytes. The levels of (c) miR-30e-3p and (d) Egr-1 mRNA measured using RT-qPCR. Overexpression of miR-30e-3p reduced Egr-1 mRNA levels, while inhibition of miR-30e-3p increased Egr-1 mRNA expression. (e) Expression of LC3II and p62 determined by western blot. (f, g) Quantification of LC3II and p62 protein levels. (h) Egr-1 expression determined by western blot. (i) Quantification of Egr-1 levels (; and , compared to the control group; and △△, compared to the IH group; and ▽▽, compared to the IH+MIR group). Con: control; NC: negative control; IH: ischemia/hypoxia; MIR: miR-30e-3p; 3-MA: 3-methyladenine.

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