Disease Mutation Study Identifies Critical Residues for Phosphatidylserine Flippase ATP11A

Figure 2

Expression analysis of WT and mutant ATP11A proteins in HEK 293T cells when co-expressed with TMEM30A. (a) Western blot analysis of ATP11A-WT, ATP11A-I80P, ATP11A-I80V, ATP11A-Y300F, and ATP11A-D913K in HEK 293T cells using anti-Flag antibody. β-Actin was used as the loading control. Uncropped gel pictures were shown in Figure S1. (b) Quantification of WT and mutant ATP11A proteins revealed that the expression level of ATP11A-Y300F was reduced. ATP11A-WT was used as control. . . The data represented . (c) Quantitative real-time PCR analysis of WT and mutant ATP11A mRNA revealed that the transcriptional level of ATP11A-Y300F mutant was reduced. ATP11A-WT was used as control. . . ns: no statistical significance. The data represented . A representative result of three independent experiments was shown for a–c.

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