Autologous Micrografts from Scalp Tissue: Trichoscopic and Long-Term Clinical Evaluation in Male and Female Androgenetic Alopecia

Figure 1

Micrograft’s preparation with the “Gentile protocol” (first and second steps). (a) Harvesting of the scalp tissues with punch biopsy (2 mm diameter). (b) Storing scalp tissues in saline solution. (c) Cutting and splitting of the fragments into strips of 2.0 × 2.0 mm (group of 3 fragments each time) sterilely through a manual splitting performed by multiple incisions with scalpel number 11 in 1.2 mL of saline for each 3 fragments. (d) Splitted strips and obtained suspension; an average 20 ml for 50 fragments was disaggregated and fragmented.