Exosomes Derived from Brain Metastatic Breast Cancer Cells Destroy the Blood-Brain Barrier by Carrying lncRNA GS1-600G8.5

Figure 1

Establishment of breast cancer cell lines with high brain metastatic capacity. (a) Schematic diagram shows the protocol for the in vivo selected brain metastatic cells. Breast cancer cells (MDA-MB-231-luc-D3H2LN, MDA231) were injected into the left ventricle of 6–8 week old female wild-type BALB/c mice. After 30 days, biological imaging was performed to observe brain metastases. Cells that had metastasized in the brains of mice were isolated and defined as MDABR1. Half of the MDABR1 cells were frozen, and half were reinjected into mice. After 30 days, biological imaging was performed to observe the brain metastasis, and metastasized cells were isolated and defined as MDABR2. MDABR2 cells were subjected to a third selection to generate MDABR3 (). (b) Bioluminescent images of MDABR3 brain metastases in mice. (c) In vitro Transwell assay showing the migration and invasion capacities of MDA231 and MDABR3 cells (, -test, ).

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